Tomato hybrid SV0594TC and parents thereof

ABSTRACT

The invention provides seed and plants of tomato hybrid SV0594TC and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid SV0594TC and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. Ser. No. 14/843,846, filed Sep.2, 2015, now U.S. Pat. No. 9,681,617, which is a divisional of U.S. Ser.No. 13/864,204, filed Apr. 16, 2013, now U.S. Pat. No. 9,179,614, whichclaims the priority of U.S. Provisional Appl. Ser. No. 61/763,454, filedFeb. 11, 2013, the entire disclosures of which are incorporated hereinby reference in their entireties.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid SV0594TC and theinbred tomato lines CHI-XF-LOEM and CHI-XF-PIAS.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of thehybrid designated SV0594TC, the tomato line CHI-XF-LOEM or tomato lineCHI-XF-PIAS. Also provided are tomato plants having all thephysiological and morphological characteristics of such a plant. Partsof these tomato plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid SV0594TCand/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of tomato hybrid SV0594TC and/ortomato lines CHI-XF-LOEM and CHI-XF-PIAS is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of tomato hybrid SV0594TC and/ortomato lines CHI-XF-LOEM and CHI-XF-PIAS. The tomato seed of theinvention may be provided as an essentially homogeneous population oftomato seed of tomato hybrid SV0594TC and/or tomato lines CHI-XF-LOEMand CHI-XF-PIAS. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, insome embodiments, seed of hybrid SV0594TC and/or tomato linesCHI-XF-LOEM and CHI-XF-PIAS may be defined as forming at least about 97%of the total seed, including at least about 98%, 99% or more of theseed. The seed population may be separately grown to provide anessentially homogeneous population of tomato plants designated SV0594TCand/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid SV0594TC and/or tomato linesCHI-XF-LOEM and CHI-XF-PIAS is provided. The tissue culture willpreferably be capable of regenerating tomato plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant, and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of the hybrid SV0594TCand/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS include those traits setforth in the tables herein. The regenerable cells in such tissuecultures may be derived, for example, from embryos, meristems,cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers,seed and stalks. Still further, the present invention provides tomatoplants regenerated from a tissue culture of the invention, the plantshaving all the physiological and morphological characteristics of hybridSV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent tomatoplants is a plant of tomato line CHI-XF-LOEM or tomato line CHI-XF-PIAS.These processes may be further exemplified as processes for preparinghybrid tomato seed or plants, wherein a first tomato plant is crossedwith a second tomato plant of a different, distinct genotype to providea hybrid that has, as one of its parents, a plant of tomato lineCHI-XF-LOEM or tomato line CHI-XF-PIAS. In these processes, crossingwill result in the production of seed. The seed production occursregardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid SV0594TC and/or tomatolines CHI-XF-LOEM and CHI-XF-PIAS. In one embodiment of the invention,tomato seed and plants produced by the process are first generation (F₁)hybrid tomato seed and plants produced by crossing a plant in accordancewith the invention with another, distinct plant. The present inventionfurther contemplates plant parts of such an F₁ hybrid tomato plant, andmethods of use thereof. Therefore, certain exemplary embodiments of theinvention provide an F₁ hybrid tomato plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SV0594TC and/or tomato linesCHI-XF-LOEM and CHI-XF-PIAS, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SV0594TC and/or tomatolines CHI-XF-LOEM and CHI-XF-PIAS, wherein said preparing comprisescrossing a plant of the hybrid SV0594TC and/or tomato lines CHI-XF-LOEMand CHI-XF-PIAS with a second plant; and (b) crossing the progeny plantwith itself or a second plant to produce a seed of a progeny plant of asubsequent generation. In further embodiments, the method mayadditionally comprise: (c) growing a progeny plant of a subsequentgeneration from said seed of a progeny plant of a subsequent generationand crossing the progeny plant of a subsequent generation with itself ora second plant; and repeating the steps for an additional 3-10generations to produce a plant derived from hybrid SV0594TC and/ortomato lines CHI-XF-LOEM and CHI-XF-PIAS. The plant derived from hybridSV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS may be aninbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid SV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid SV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS, whereinthe plant has been cultivated to maturity, and (b) collecting at leastone tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid SV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIASis provided. The phrase “genetic complement” is used to refer to theaggregate of nucleotide sequences, the expression of which sequencesdefines the phenotype of, in the present case, a tomato plant, or a cellor tissue of that plant. A genetic complement thus represents thegenetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SV0594TC and/or tomato lines CHI-XF-LOEMand CHI-XF-PIAS could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and areincluded to further demonstrate certain aspects of the presentinvention. The invention may be better understood by reference to one ormore of these drawings in combination with the detailed description ofspecific embodiments presented herein.

FIGS. 1 (a-d): Mean of TYLCV score of the different hybrids in the fourconducted trials. Trial 1: East Almería, Trial 2: Albuñol(Almería-Granada), Trial 3: El Ejido station and Trial 4: Beit-Yosef(Israel). Each plant was scored for TYLCV symptoms using a scale between1-9.

FIGS. 2 (a-b): Performance data of hybrid SV0594TC and ComparativeVarieties

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of tomato hybrid SV0594TC, tomato line CHI-XF-LOEMand tomato line CHI-XF-PIAS.

Tomato hybrid SV0594TC, also known as 11-XF-CCT-0594, is a highlyproductive round-fruited cherry tomato variety for loose harvest. HybridSV0594TC is highly productive because of multi-branching clusters. Thefruits have a light greenback, have intense red color, and are tolerantto cracking. SV0594TC plants have dark green leaves, are very vigorousand especially suitable for long crops (>20 clusters). SV0594TC has avery high level of resistance to the TYLCV pathogen, higher thancommercial varieties known to date.

Tomato hybrid SV0594TC is especially suitable for long crops growingthrough low temperature and high humidity conditions during wintercropping. Because of the high level of resistance of SV0594TC to TYLCV,the variety is especially suitable to be grown in areas where thisdisease is known to be a recurring problem.

A. Origin and Breeding History of Tomato Hybrid SV0594TC

The parents of hybrid SV0594TC are CHI-XF-LOEM and CHI-XF-PIAS. Theseparents were created as follows:

Tomato line CHI-XF-LOEM was created as follows:

-   -   F2(07MG0220)-3→F3(08SP0072)-20→F4(09SP0051)-4→F5(09SP0377)-3    -   F6(10SP0127)-9→F7(11SP0015)=CHI-XF-LOEM

Tomato line CHI-XF-PIAS was created as follows:

-   -   F1(F7[04SP0191]×F2[04SP0026])=05SP0074-1→F2(05SP0333)-1→F3(06SP0052)-10→F4(07SP0026)-2→F5(07SP0202)-18→F6(08SP0105)-2→F7(09SP0225)=F7(09SP0525)-10→F8(10SP0041)−29→F9(11SP0025)=CHI-XF-PIAS

Hybrid SV0594TC was created as follows:

SV0594TC=F1(CHI-XF-LOEM X CHI-XF-PIAS)

The parent lines are uniform and stable, as is a hybrid producedtherefrom. A small percentage of variants can occur within commerciallyacceptable limits for almost any characteristic during the course ofrepeated multiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Tomato HybridSV0594TC, Tomato Line CHI-XF-LOEM and Tomato Line CHI-XF-PIAS

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of tomato hybrid SV0594TC and the parent lines thereof.A description of the physiological and morphological characteristics ofsuch plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSV0594TC Comparison: Characteristic SV0594TC Katalina 1. Seedlinganthocyanin in hypocotyl of 2-15 cm present present seedling (MontfavetH 63.4) (Montfavet H 63.4) habit of 3-4 week old seedling normal normal2. Mature Plant height 268.66 cm 256.13 cm growth type indeterminateindeterminate (Marmande VR, (Marmande VR, Saint-Pierre, SanSaint-Pierre, San Marzano 2) Marzano 2) form lax, open normal normallax, open size of canopy (compared to others of large large similartype) habit sprawling sprawling (decumbent) (decumbent) 3. Stemanthocyanin coloration of upper third absent or very weak absent or veryweak Length of internode (between 1^(st) and short (Dombito, medium4^(th) inflorescence) (only indeterminate Manific, Paso, Trend)(Montfavet H 63.5) growth type varieties) branching intermediateintermediate (Westover); (Westover) Profuse (UC 82) branching atcotyledon or first leafy present present node number of nodes betweenfirst 7 to 10 7 to 10 inflorescence number of nodes between early(1^(st) to 1 to 4  1 to 4  2^(nd), 2^(nd) to 3^(rd)) inflorescencesnumber of nodes between later 1 to 4  1 to 4  developing inflorescencespubescence on younger stems sparsely hairy sparsely hairy (scatteredlong hairs) (scattered long hairs) 4. Leaf type (mature leaf beneath the3^(rd) tomato tomato inflorescence) margins of major leaflets (matureleaf nearly entire shallowly toothed beneath the 3^(rd) inflorescence)or scalloped marginal rolling or wiltiness (mature slight slight leafbeneath the 3^(rd) inflorescence) onset of leaflet rolling early seasonmid season; late season surface of major leaflets (mature leaf rugosesmooth beneath the 3^(rd) inflorescence) (bumpy or veiny) pubescence(mature leaf beneath the smooth smooth 3^(rd) inflorescence) (no longhairs) (no long hairs) attitude (in middle third of plant) horizontal(Aromata, Triton) length medium (Lorena) medium (Lorena) width mediummedium division of blade bipinnate (Lukullus, bipinnate (Lukullus,Saint-Pierre) Saint-Pierre) size of leaflets (in middle of leaf) medium(Marmande medium (Marmande VR, Royesta) VR, Royesta) intensity of greencolor medium (Lucy) medium (Lucy) glossiness weak (Daniela) weak(Daniela) blistering weak (Daniela) strong (Delfine, Tiny Tim) size ofblisters medium large (Marmande VR) (Daniela, Egéris) attitude ofpetiole of leaflet in relation semi-erect (Blizzard, semi-erect(Blizzard, to main axis (in middle of leaf) Marmande VR) Marmande VR);horizontal (Sonatine) 5. Inflorescence inflorescence type (2^(nd) and3^(rd) truss) intermediate mainly uniparous (Harzfeuer) (Dynamo) type(3^(rd) inflorescence) forked (2 major axes) simple average number offlowers in 19 17.86 inflorescence (3^(rd) inflorescence) leafy or“running” inflorescence (3^(rd) absent absent inflorescence) 6. Flowercalyx normal normal (lobes awl shaped) (lobes awl shaped) calyx-lobesshorter than corolla distinctly longer than corolla corolla color yellowyellow style pubescence sparse absent or very scarce (Campbell 1327)anthers all fused into tube all fused into tube fasciation (1^(st)flower of 2^(nd) or 3^(rd) absent (Monalbo, absent (Monalbo,inflorescence) Moneymaker) Moneymaker) color yellow yellow (Marmande VR)(Marmande VR) 7. Fruit typical shape in longitudinal section circularcircular (3^(rd) fruit of 2^(nd) or 3^(rd) cluster) shape oftransverse/cross section (3^(rd) round round fruit of 2^(nd) or 3^(rd)cluster) shape of stem end (3^(rd) fruit of 2^(nd) or 3^(rd) flat flatcluster) shape of blossom end (3^(rd) fruit of 2^(nd) or flat (Montfavetflat (Montfavet 3^(rd) cluster) H 63.4, Montfavet H 63.4, Montfavet H63.5) H 63.5) size of blossom scar very small (Cerise, very small(Cerise, Early Mech, Early Mech, Europeel, Heinz Europeel, Heinz 1706,Peto Gro, Rio 1706, Peto Gro, Rio Grande) Grande) shape of pistil scar(3^(rd) fruit of 2^(nd) or Dot Dot 3^(rd) cluster) peduncle: abscissionlayer (3^(rd) fruit of present (pedicellate) present (pedicellate)2^(nd) or 3^(rd) cluster) (Montfavet H 63.5, Roma peduncle: length (fromabscission layer medium (Dario, short (Cerise, to calyx) (only forvarieties with Primosol) Ferline, Montfavet abscission layers) H 63.18,Rossol) ribbing at peduncle end absent or very weak absent or very weak(Calimero, Cerise) (Calimero, Cerise) depression at peduncle end absentor very weak absent or very weak (Europeel, Heinz (Europeel, Heinz 1706,Rossol, Sweet 1706, Rossol, Sweet Baby) Baby) size of stem/peduncle scarsmall (Early Mech, small (Early Mech, Peto Gro, Rio Peto Gro, RioGrande, Roma) Grande, Roma) point of detachment of fruit at harvest atcalyx attachment at pedicel joint (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) length of dedicel (3^(rd) fruit of 2^(nd) or 3^(rd) 26.80 mm23.37 mm cluster) length of mature fruit (stem axis; 3^(rd) 32.18 mm33.68 mm fruit of 2^(nd) or 3^(rd) cluster) diameter of fruit at widestpoint (3^(rd) 33.63 mm 32.49 mm fruit of 2^(nd) or 3^(rd) cluster)weight of mature fruit (3^(rd) fruit of 2^(nd) 22.53 grams 22.66 gramsor 3^(rd) cluster) size small (Early Mech, small (Early Mech, Europeel,Roma) Europeel, Roma) ratio length/diameter medium (Early medium (EarlyMech, Peto Gro) Mech, Peto Gro) core present present size of core incross section (in relation very small (Cerise) very small (Cerise) tototal diameter) number of locules only 2 (Early Mech, only 2 (EarlyMech, Europeel, San Europeel, San Marzano) Marzano) surface smoothsmooth base color (mature-green stage) apple or medium apple or mediumgreen (Heinz green (Heinz 1439 VF) 1439 VF) pattern (mature-green stage)green-shouldered green-shouldered green shoulder (before maturity)present (Daniela, present (Daniela, Montfavet H 63.5) Montfavet H 63.5)shoulder color if different from base dark green dark green extent ofgreen shoulder (before small (Cristy, small (Cristy, maturity)Firestone) Firestone) intensity of green color of shoulder dark (Ayala,Erlidor, dark (Ayala, Erlidor, (before maturity) Xenon) Xenon) intensityof green color of fruit (before medium (Rody) medium (Rody) maturity)color at maturity (full-ripe) red (Ferline, red (Ferline, Daniela,Montfavet Daniela, Montfavet H 63.5) H 63.5) color of flesh at maturity(full-ripe) red/crimson red/crimson (Ferline, Saint-Pierre) (Ferline,Saint-Pierre) flesh color with lighter and with lighter and darker areasin walls darker areas in walls locular gel color of table-ripe fruityellow yellow firmness soft (Trend) soft (Trend) shelf life long(Daniela) long (Daniela) time of flowering early (Feria, early (Feria,Primabel) Primabel) time of maturity early (Feria, Rossol) early (Feria,Rossol) ripening (blossom-to-stem axis) uniform blossom-to-stem endripening (peripheral to central radial outside in outside in axis)epidermis color yellow yellow epidermis easy-peel easy-peel epidermistexture average average thickness of pericarp medium (Carmello, medium(Carmello, Europeel, Floradade, Europeel, Floradade, Heinz 1706, Heinz1706, Montfavet H 63.5) Montfavet H 63.5) dry matter content (atmaturity) high (Aloha, medium Coudoulet) 8. Chemistry and Composition ofFull-Ripe Fruits pH 4.7 4.2 titratable acidity, as % citric 6.62 9.63total solids (dry matter, seeds and skin 8.15 7.96 removed) (percentagetotal content) soluble Solids as °Brix 6.9 8.3 9. Phenology seeding to50% flow (1 open on 50% 49 days of plants) fruiting season long(Marglobe) 10. Adaptation culture greenhouse principle use(s) freshmarket machine harvest not adapted *These are typical values. Values mayvary due to environment. Other values that are substantially equivalentare also within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of LineCHI-XF-LOEM Characteristic CHI-XF-LOEM 1. Seedling anthocyanin inhypocotyl of present 2-15 cm seedling (Montfavet H 63.4) habit of 3-4week old seedling normal 2. Mature Plant height 218.66 cm growth typeindeterminate (Marmande VR, Saint-Pierre, San Marzano 2) form lax, opennormal size of canopy (compared to medium others of similar type) habitsemi-erect 3. Stem anthocyanin coloration of absent or very weak upperthird Length of internode (between short (Dombito, Manific, 1^(st) and4^(th) inflorescence) Paso, Trend) (only indeterminate growth typevarieties) branching intermediate (Westover) branching at cotyledonpresent or first leafy node number of nodes between 7 to 10 firstinflorescence number of nodes between 1 to 4  early (1^(st) to 2^(nd),2^(nd) to 3^(rd)) inflorescences number of nodes between later 1 to 4 developing inflorescences pubescence on younger stems sparsely hairy(scattered long hairs) 4. Leaf type (mature leaf beneath the tomato3^(rd) inflorescence) margins of major leaflets deeply toothed or cut,(mature leaf beneath the 3^(rd) sps. toward base inflorescence) marginalrolling or wiltiness absent (mature leaf beneath the 3^(rd)inflorescence) onset of leaflet rolling late season surface of majorleaflets rugose (mature leaf beneath the 3^(rd) (bumpy or veiny)inflorescence) pubescence (mature leaf normal beneath the 3^(rd)inflorescence) attitude (in middle third horizontal of plant) (Aromata,Triton) length medium (Lorena) width narrow (Marmande VR, Red Robin,Tiny Tim) division of blade bipinnate (Lukullus, Saint-Pierre) size ofleaflets (in middle of leaf) small (Tiny Tim) intensity of green colormedium (Lucy) glossiness medium (Marmande VR) blistering strong(Delfine, Tiny Tim) size of blisters medium (Marmande VR) attitude ofpetiole of leaflet in horizontal (Sonatine) relation to main axis (inmiddle of leaf) 5. Inflorescence inflorescence type intermediate (2^(nd)and 3^(rd) truss) (Harzfeuer) type (3^(rd) inflorescence) forked (2major axes) average number of flowers in 28.8 inflorescence (3^(rd)inflorescence) leafy or “running” inflorescence absent (3^(rd)inflorescence) 6. Flower calyx normal (lobes awl shaped) calyx-lobesshorter than corolla corolla color yellow style pubescence absent orvery scarce (Campbell 1327) anthers all fused into tube fasciation(1^(st) flower of 2^(nd) or 3^(rd) absent inflorescence) (Monalbo,Moneymaker) color yellow (Marmande VR) 7. Fruit typical shape inlongitudinal section circular (3^(rd) fruit of 2^(nd) or 3^(rd) cluster)shape of transverse/cross section round (3^(rd) fruit of 2^(nd) or3^(rd) cluster) shape of stem end (3^(rd) fruit of flat 2^(nd) or 3^(rd)cluster) shape of blossom end (3^(rd) fruit flat (Montfavet H 63.4, of2^(nd) or 3^(rd) cluster) Montfavet H 63.5) size of blossom scar verysmall (Cerise, Early Mech, Europeel, Heinz 1706, Peto Gro, Rio Grande)peduncle: abscission layer present (pedicellate) (3^(rd) fruit of 2^(nd)or 3^(rd) cluster) (Montfavet H 63.5, Roma peduncle: length (fromabscission short (Cerise, layer to calyx) (only for varieties Ferline,Montfavet with abscission layers) H 63.18, Rossol) ribbing at peduncleend absent or very weak (Calimero, Cerise) depression at peduncle endabsent or very weak (Europeel, Heinz 1706, Rossol, Sweet Baby) size ofstem/peduncle scar very small (Cerise, Heinz 1706, Sweet Baby) point ofdetachment of fruit at pedicel joint at harvest (3^(rd) fruit of 2^(nd)or 3^(rd) cluster) length of dedicel (3^(rd) fruit of 20.38 mm 2^(nd) or3^(rd) cluster) length of mature fruit 29.88 mm (stem axis; 3^(rd) fruitof 2^(nd) or 3^(rd) cluster) diameter of fruit at widest 30.10 mm point(3^(rd) fruit of 2^(nd) or 3^(rd) cluster) weight of mature fruit(3^(rd) 17.2 grams fruit of 2^(nd) or 3^(rd) cluster) size small (EarlyMech, Europeel, Roma) ratio length/diameter medium (Early Mech, PetoGro) core present size of core in cross section small (Early Mech, (inrelation to total diameter) Europeel, Heinz 1706, Peto Gro, Rio Grande,Rossol) number of locules only 2 (Early Mech, Europeel, San Marzano)surface smooth base color (mature-green stage) light green (Lanai, VF145-F5); apple or medium green (Heinz 1439 VF) pattern (mature-greenstage) green-shouldered green shoulder (before maturity) present(Daniela, Montfavet H 63.5) shoulder color if different dark green frombase extent of green shoulder medium (Erlidor, Foxy, (before maturity)Montfavet H 63.5) intensity of green color of dark (Ayala, shoulder(before maturity) Erlidor, Xenon) intensity of green color of fruitlight (Capello, (before maturity) Duranto, Trust) color at maturity(full-ripe) red (Ferline, Daniela, Montfavet H 63.5) color of flesh atmaturity red/crimson (Ferline, (full-ripe) Saint-Pierre) flesh colorwith lighter and darker areas in walls locular gel color of table-yellow ripe fruit firmness soft (Trend) shelf life medium (Durinta) timeof flowering early (Feria, Primabel) time of maturity early (Feria,Rossol) ripening (blossom-to- uniform stem axis) ripening (peripheral touniformity central radial axis) epidermis color colorless epidermiseasy-peel epidermis texture average thickness of pericarp thin (MarmandeVR) 8. Chemistry and Composition of Full-Ripe Fruits pH 4.5 titratableacidity, as % citric 5.8 soluble Solids as °Brix 5.5 9. Phenologyseeding to 50% flow 41 days (1 open on 50% of plants) 10. Adaptationculture greenhouse principle use(s) fresh market machine harvest notadapted *These are typical values. Values may vary due to environment.Other values that are substantially equivalent are also within the scopeof the invention.

TABLE 3 Physiological and Morphological Characteristics of LineCHI-XF-PIAS Characteristic CHI-XF-PIAS 1. Seedling anthocyanin inhypocotyl of present 2-15 cm seedling (Montfavet H 63.4) habit of 3-4week old seedling normal 2. Mature Plant height 201 cm growth typeindeterminate (Marmande VR, Saint-Pierre, San Marzano 2) form lax, opensize of canopy (compared to medium others of similar type) habitsemi-erect 3. Stem anthocyanin coloration of absent or very weak upperthird Length of internode (between medium 1^(st) and 4^(th)inflorescence) (Montfavet H 63.5) (only indeterminate growth typevarieties) branching profuse (UC 82) branching at cotyledon or presentfirst leafy node number of nodes between 7 to 10 first inflorescencenumber of nodes between 1 to 4  early (1^(st) to 2^(nd), 2^(nd) to3^(rd)) inflorescences number of nodes between later 1 to 4  developinginflorescences pubescence on younger stems smooth (no long hairs);sparsely hairy (scattered long hairs) 4. Leaf type (mature leaf beneathtomato the 3^(rd) inflorescence) margins of major leaflets deeplytoothed or cut, (mature leaf beneath the 3^(rd) sps. toward baseinflorescence) marginal rolling or wiltiness strong (mature leaf beneaththe 3^(rd) inflorescence) surface of major leaflets smooth (mature leafbeneath the 3^(rd) inflorescence) pubescence (mature leaf normal beneaththe 3^(rd) inflorescence) attitude (in middle third semi-erect(Allround, of plant) Drakar, Vitador) length short (Nelson, Red Robin,Tiny Tim) width narrow (Marmande VR, Red Robin, Tiny Tim) division ofblade bipinnate (Lukullus, Saint-Pierre) size of leaflets (in middlesmall (Tiny Tim) of leaf) intensity of green color light (Macero II,Poncette, Rossol) glossiness weak (Daniela) blistering weak (Daniela)size of blisters small (Husky Cherrie Red) attitude of petiole ofleaflet horizontal (Sonatine) in relation to main axis (in middle ofleaf) 5. Inflorescence inflorescence type (2^(nd) and mainly multiparous3^(rd) truss) (Marmande VR) type (3^(rd) inflorescence) compound (muchbranched) average number of flowers 56 in inflorescence (3^(rd)inflorescence) leafy or “running” inflorescence absent (3^(rd)inflorescence) 6. Flower calyx normal (lobes awl shaped) calyx-lobesshorter than corolla corolla color yellow style pubescence absent orvery scarce (Campbell 1327); sparse anthers all fused into tubefasciation (1^(st) flower of 2^(nd) or absent 3^(rd) inflorescence)(Monalbo, Moneymaker) color yellow (Marmande VR) 7. Fruit typical shapein longitudinal circular section (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) shape of transverse/cross round section (3^(rd) fruit of 2^(nd)or 3^(rd) cluster) shape of stem end (3^(rd) fruit flat of 2^(nd) or3^(rd) cluster) shape of blossom end (3^(rd) fruit flat (Montfavet H63.4, of 2^(nd) or 3^(rd) cluster) Montfavet H 63.5) size of blossomscar very small (Cerise, Early Mech, Europeel, Heinz 1706, Peto Gro, RioGrande) peduncle: abscission layer present (pedicellate) (3^(rd) fruitof 2^(nd) or 3^(rd) cluster) (Montfavet H 63.5, Roma peduncle: length(from medium abscission layer to calyx) (Dario, Primosol) (only forvarieties with abscission layers) ribbing at peduncle end absent or veryweak (Calimero, Cerise) depression at peduncle end absent or very weak(Europeel, Heinz 1706, Rossol, Sweet Baby) length of dedicel (3^(rd)fruit 21.09 mm of 2^(nd) or 3^(rd) cluster) length of mature fruit 30.76mm (stem axis; 3^(rd) fruit of 2^(nd) or 3^(rd) cluster) diameter offruit at widest 30.15 mm point (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) weight of mature fruit 16.86 mm (3^(rd) fruit of 2^(nd) or3^(rd) cluster) size small (Early Mech, Europeel, Roma) ratiolength/diameter medium (Early Mech, Peto Gro) core present size of corein cross section small (Early Mech, (in relation to total diameter)Europeel, Heinz 1706, Peto Gro, Rio Grande, Rossol) number of loculesonly 2 (Early Mech, Europeel, San Marzano) surface smooth base color(mature-green stage) light green (Lanai, VF 145-F5); apple or mediumgreen (Heinz 1439 VF) pattern (mature-green stage) green-shoulderedgreen shoulder (before present (Daniela, maturity) Montfavet H 63.5)shoulder color if different dark green from base extent of greenshoulder medium (Erlidor, Foxy, (before maturity) Montfavet H 63.5)intensity of green color of medium shoulder (before maturity) (MontfavetH 63.5) intensity of green color of light (Capello, fruit (beforematurity) Duranto, Trust) color at maturity (full-ripe) orange (Sungold)color of flesh at maturity red/crimson (full-ripe) (Ferline,Saint-Pierre) flesh color with lighter and darker areas in walls loculargel color of table- yellow ripe fruit firmness medium (Cristina) shelflife very long (Ernesto) time of flowering early (Feria, Primabel) timeof maturity late (Manific, Saint-Pierre) ripening (blossom-to- uniformstem axis) ripening (peripheral to central uniformity radial axis)epidermis color yellow epidermis normal epidermis texture averagethickness of pericarp thin (Marmande VR) 8. Chemistry and Composition ofFull-Ripe Fruits pH 4.1 titratable acidity, as % citric 13.1 solubleSolids as °Brix 6.7 9. Phenology seeding to 50% flow 49 days (1 open on50% of plants) 10. Adaptation culture greenhouse principle use(s) freshmarket machine harvest not adapted *These are typical values. Values mayvary due to environment. Other values that are substantially equivalentare also within the scope of the invention.

C. Breeding Tomato Plants

One aspect of the current invention concerns methods for producing seedof tomato hybrid SV0594TC involving crossing tomato lines CHI-XF-LOEMand CHI-XF-PIAS. Alternatively, in other embodiments of the invention,hybrid SV0594TC, line CHI-XF-LOEM, or line CHI-XF-PIAS may be crossedwith itself or with any second plant. Such methods can be used forpropagation of hybrid SV0594TC and/or the tomato lines CHI-XF-LOEM andCHI-XF-PIAS, or can be used to produce plants that are derived fromhybrid SV0594TC and/or the tomato lines CHI-XF-LOEM and CHI-XF-PIAS.Plants derived from hybrid SV0594TC and/or the tomato lines CHI-XF-LOEMand CHI-XF-PIAS may be used, in certain embodiments, for the developmentof new tomato varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SV0594TC followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSV0594TC and/or tomato lines CHI-XF-LOEM and CHI-XF-PIAS for the purposeof developing novel tomato lines, it will typically be preferred tochoose those plants which either themselves exhibit one or more selecteddesirable characteristics or which exhibit the desired characteristic(s)when in hybrid combination. Examples of desirable traits may include, inspecific embodiments, high seed yield, high seed germination, seedlingvigor, high fruit yield, disease tolerance or resistance, andadaptability for soil and climate conditions. Consumer-driven traits,such as a fruit shape, color, texture, and taste are other examples oftraits that may be incorporated into new lines of tomato plantsdeveloped by this invention.

D. Performance Characteristics

As described above, hybrid SV0594TC exhibits desirable traits, asconferred by tomato lines CHI-XF-LOEM and CHI-XF-PIAS. The performancecharacteristics of hybrid SV0594TC and tomato lines CHI-XF-LOEM andCHI-XF-PIAS were the subject of an objective analysis of the performancetraits relative to other varieties. The results of the analysis arepresented in FIG. 1 (a-d) and FIG. 2 (a-b).

E. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

F. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

G. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS color chart byidentifying the group name, sheet number and letter, e.g., Yellow-OrangeGroup 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a tomatovariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation.

H. Deposit Information

A deposit of tomato hybrid SV0594TC and inbred parent lines CHI-XF-LOEMand CHI-XF-PIAS, disclosed above and recited in the claims, has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The date of deposits was Mar. 4, 2013.The accession numbers for those deposited seeds of tomato hybridSV0594TC and inbred parent lines CHI-XF-LOEM and CHI-XF-PIAS are ATCCAccession No. PTA-13586, ATCC Accession No. PTA-13585, and ATCCAccession No. PTA-13587, respectively. Upon issuance of a patent, allrestrictions upon the deposits will be removed, and the deposits areintended to meet all of the requirements of 37 C.F.R. § 1.801-1.809. Thedeposits will be maintained in the depository for a period of 30 years,or 5 years after the last request, or for the effective life of thepatent, whichever is longer, and will be replaced if necessary duringthat period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A tomato plant of tomato hybrid SV0594TC, asample of seed of said hybrid SV0594TC having been deposited under ATCCAccession Number PTA-13586.
 2. A tomato seed that produces the plant ofclaim
 1. 3. A plant part of the plant of claim 1, wherein said plantpart comprises a cell of said plant.
 4. The plant part of claim 3,wherein said plant part comprises a leaf, an ovule, pollen, a fruit, ora cell.
 5. A tissue culture of regenerable cells of the plant ofclaim
 1. 6. The tissue culture according to claim 5, comprising cells orprotoplasts from a plant part selected from the group consisting ofembryos, meristems, cotyledons, pollen, leaves, anthers, roots, roottips, pistils, flowers, seeds, and stalks, wherein said cells orprotoplasts comprise a cell or protoplast of said tomato hybridSV0594TC.
 7. A tomato plant regenerated from the tissue culture of claim5, wherein said regenerated plant comprises all of the physiological andmorphological characteristics of said tomato hybrid SV0594TC.
 8. Amethod of vegetatively propagating the tomato plant of claim 1, themethod comprising the steps of: (a) collecting tissue capable of beingpropagated from the plant according to claim 1; (b) cultivating saidtissue to obtain proliferated shoots; and (c) rooting said proliferatedshoots to obtain a rooted plantlet.
 9. The method of claim 8, furthercomprising growing at least a first tomato plant from said rootedplantlet.
 10. A method of producing a tomato plant comprising an addedtrait, the method comprising introducing a transgene conferring thetrait into the plant of claim
 1. 11. A tomato plant produced by themethod of claim 10, wherein said plant comprises said trait andotherwise comprises all of the morphological and physiologicalcharacteristics of said tomato hybrid SV0594TC.
 12. A tomato plant oftomato hybrid SV0594TC, a sample of seed of said hybrid having beendeposited under ATCC Accession Number PTA-13586, further comprising atransgene.
 13. The plant of claim 12, wherein the transgene confers atrait selected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism and modified protein metabolism.
 14. A tomatoplant of tomato hybrid SV0594TC, a sample of seed of said hybrid havingbeen deposited under ATCC Accession Number PTA-13586, further comprisinga single locus conversion.
 15. The plant of claim 14, wherein the singlelocus conversion confers a trait selected from the group consisting ofmale sterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism and modified proteinmetabolism.
 16. A method for producing a seed of a tomato plant derivedfrom tomato hybrid SV0594TC, the method comprising the steps of: (a)crossing the tomato plant of claim 1 with itself or a second tomatoplant; and (b) allowing a seed of a hybrid SV0594TC-derived tomato plantto form.
 17. The method of claim 16, further comprising the steps of:(c) selfing a plant grown from said hybrid SV0594TC-derived tomato seedto yield an additional hybrid SV0594TC-derived tomato seed; (d) growingsaid additional hybrid SV0594TC-derived tomato seed of step (c) to yieldan additional hybrid SV0594TC-derived tomato plant; and (e) repeatingthe selfing and growing steps of (c) and (d) to generate at least afirst further hybrid SV0594TC-derived tomato plant.
 18. The method ofclaim 16, wherein the second tomato plant is of an inbred tomato line.19. The method of claim 17, further comprising: (f) crossing the furtherhybrid SV0594TC-derived tomato plant with a different tomato plant toproduce a seed of a progeny plant.
 20. A method of producing a tomatofruit, the method comprising: (a) obtaining the plant according to claim1, wherein the plant has been cultivated to maturity; and (b) collectinga tomato fruit from the plant.